DNA identification of Zijingpi's adulterant species Schisandra sphenanthera based on NCBI nucleotide database analysis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2534-2537, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-263892
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To provide basis for quality control of Zijingpi, DNA identification was used based on NCBI nucleotide database analysis.</p><p><b>METHOD</b>Firstly, total DNA of Zijingpi was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR products was directly sequenced after purification. Finally, ITS sequence similarity and phylogenetic tree were used for identification.</p><p><b>RESULT</b>The ITS sequence information of the mainstream commercial drugs of Zijingpi was obtained.</p><p><b>CONCLUSION</b>It is firstly reported that the mainstream commercial drugs of Zijingpi was the bark of Schisandra sphenanthera.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Controle de Qualidade
/
Padrões de Referência
/
Medicamentos de Ervas Chinesas
/
Dados de Sequência Molecular
/
Química
/
Classificação
/
Análise de Sequência de DNA
/
DNA de Plantas
/
Bases de Dados de Ácidos Nucleicos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo