Nanog promotes the invasion of breast cancer cells by increasing PKCε expression / 南方医科大学学报
Journal of Southern Medical University
; (12): 639-644, 2016.
Article
em Zh
| WPRIM
| ID: wpr-263989
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between Nanog-promoted metastasis of breast cancer and ezrin(T567) phosphorylation, and explore the possible mechanism by which Nanog regulates ezrin(T567) phosphorylation.</p><p><b>METHODS</b>A siRNA construct targeting Nanog was transfected in breast cancer cells to knock down Nanog expression, and the changes in the cell invasion was detected using Transwell assay. The expression levels of Nanog and PKC and the phosphorylation level of ezrin(T567) were detected using Western blotting and immunofluorescent staining; the protein interaction between PKCε and ezrin was assayed by co-immunoprecipitation and Western blotting.</p><p><b>RESULTS</b>Nanog knockdown significantly decreased the expression of PKCε protein, phosphorylation level of ezrin(T567) and the invasion ability of breast cancer cells. PKCε knockdown obviously decreased the phosphorylation level of ezrin(T567) in the cells, and PKCε and ezrin were co-immunoprecipitated.</p><p><b>CONCLUDIONS</b>Nanogcan can upregulate the expression of PKCε to promote the phosphorylation of ezrin(T567), which can be a new mechanism by which Nanog promotes tumor metastasis.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fosforilação
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Neoplasias da Mama
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Células Tumorais Cultivadas
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Transfecção
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Regulação para Cima
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Western Blotting
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Proteínas de Homeodomínio
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Proteínas do Citoesqueleto
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RNA Interferente Pequeno
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Proteína Quinase C-épsilon
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Article