Expression of CD14 and Toll-like receptor 4 on Kupffer cells and its role in ischemia-reperfusion injury on rat liver graft / 中华外科杂志
Chinese Journal of Surgery
; (12): 274-276, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-264526
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of lipopolysaccharide (LPS) receptor CD14 and Toll-like receptor 4 (TLR4) on Kupffer cells and its role in ischemia-reperfusion injury (IRI) on rat liver graft.</p><p><b>METHODS</b>The Kupffer cells following IRI were isolated and divided into control, ischemia-reperfusion (IR), and anti-CD14 antibody group. The mRNA and protein expression of CD14 and TLR4, nuclear factor kappa B (NF-kappaB) activity and TNF-alpha level in supernatant were measured.</p><p><b>RESULTS</b>The mRNA and protein expression of CD14 and TLR4 in IR group were significantly higher than those in control group (P < 0.01). The NF-kappaB activity and TNF-alpha level in IR group were significantly higher than those in control group (P < 0.01), and they greatly decreased after anti CD14 antibody treatment (compared with IR group, P < 0.05), but were still significantly higher than those in control group (P < 0.01).</p><p><b>CONCLUSIONS</b>LPS following IRI could up-regulate CD14 and TLR4 gene and protein expression on Kupffer cells, and subsequently activate NF-kappaB to produce cytokines, but other signal transduction pathways might also participate in the IRI.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Técnicas In Vitro
/
RNA Mensageiro
/
Traumatismo por Reperfusão
/
Transdução de Sinais
/
NF-kappa B
/
Transplante de Fígado
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Fator de Necrose Tumoral alfa
/
Ratos Wistar
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2005
Tipo de documento:
Artigo