Anti-emetic effect of ethanol extract from "Wuzhuyu broth" / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the anti-emetic effect of ethanol extract from "WuZhuYu broth" and its mechanism.</p><p><b>METHOD</b>Three experiments were carried out test which extract has anti-emetic activity, such as CuSO4-induced pigeon's emetic response, gastric emptying in mice and ACh-induced or 5-HT-induced contraction in vitro gastric muscle in rats. Meanwhile, effect of anti-emetic extract on concentration-response curve to ACh, 5-HT, histamine was investigated.</p><p><b>RESULT</b>50% ethanol extract and 70% ethanol extract were identified as having significantly stronger anti-emetic activities with little side effect, which showed the significant effect on concentration-response curve to ACh, 5-HT, histamine.</p><p><b>CONCLUSION</b>50% ethanol extract and 70% ethanol extract contain more anti-emetic fractions, more anti-emetic fractions can be gained at the concentrations of 50% and 70% ethanol; the mechanism of anti-emetic effect is related to its antagonism to the receptors of ACh, 5-HT, histamine.</p>