Reconstruction and expression of green fluorescent protein and aquaporin 7 fusion recombinant vector / 中华男科学杂志
National Journal of Andrology
; (12): 819-823, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-267806
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the membrane mobility of aquaporin 7 (AQP7) by cloning stably transfected CHO cells with expression of pEGFP-C1-AQP7, in which AQP7 cDNA was fused downstream and in frame to pEGFP-C1 gene.</p><p><b>METHODS</b>The full sequence of AQP7 was amplified by RT-PCR and then recombined in the downstream of the green fluorescent protein gene in the pEGFP-C1 vector. The recombinant vector pEGFP-C1-AQP7 was stably transfected into CHO cells. With fluorescent microscopy, immunocytochemical stain and Western blot, pEGFP-C1-AQP7 showed a predominant intracellular vesicular localization.</p><p><b>RESULTS</b>(1) The sequence of AQP7 cDNA of the Wistar rat was logged into the GenBank (access number AY157737). (2) Identification demonstrated that pEGFP-C1-AQP7 fusion protein stably expressed in CHO cells. (3) With fluorescence microscopy, pEGFP-C1-AQP7 showed a predominant intracellular vesicular localization.</p><p><b>CONCLUSION</b>The CHO cell line with stable pEGFP-C1-AQP7 expression was set up successfully for advanced research.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Testículo
/
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
/
Transfecção
/
Cricetulus
/
Células CHO
/
Ratos Wistar
/
DNA Complementar
/
Aquaporinas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2004
Tipo de documento:
Artigo