The extracellular signal-regulated kinase was promoted by pyrroloquinoline quinine in cultured Schwann cells / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 444-447, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-268660
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of mitogen-activated protein kinase (MEK) kinase cascade, extracellular signal-regulated kinase (ERK1/2) signal pathway on Schwann cells proliferation promoted by Pyrroloquinoline Quinine (PQQ) and its molecular mechanisms.</p><p><b>METHODS</b>Schwann cells were cultured and purified in vitro. The purity was identified by S-100. Different time and concentration of PQQ was added into culture medium. The expression of ERK1/2 and phosphorylated-ERK1/2 was detected by western blot. The expression of p-ERK1/2 after blocking of MEK signal pathway by specific inhibitor PD98059 was detected by western blot.</p><p><b>RESULTS</b>Morphological change was observed in PQQ treated Schwann cells. 1-500 nmol/L PQQ could up-regulate the expression of p-ERK1/2, and 1000 nmol/L had no effects, while 10 000 nmol/L exhibited inhibitory effect (P < 0.05). p-ERK1/2 increased to peak 1 h after PQQ added, and this up-regulation of p-ERK1/2 was inhibited by PD98059 (P < 0.05).</p><p><b>CONCLUSIONS</b>PQQ could affect morphology of Schwann cells and activation of ERK1/2. MEK inhibitor PD98059 could, block this activation. It suggests that MEK/ERK signal pathway should be involved in Schwann cells proliferation promoted by PQQ.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Pirróis
/
Quinolinas
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Células de Schwann
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Transdução de Sinais
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Células Cultivadas
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Ratos Sprague-Dawley
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Proteínas Quinases Ativadas por Mitógeno
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Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo