Effect of FK506 on cytokine secretion in whole blood / 南方医科大学学报
Journal of Southern Medical University
; (12): 249-251, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-269581
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of FK506 on cytokine secretions in whole blood from healthy individuals.</p><p><b>METHODS</b>Blood samples collected from healthy volunteers were co-cultured with different concentrations of FK506 and stimulated with PMA and IONO. The concentrations of 8 cytokines including IL-2, IL-6, IL-12, IL-17, IFN-gamma, TNF-alpha, GM-CSF and G-CSF were detected by Bio-Plex suspension system.</p><p><b>RESULTS</b>Compared with the control group, high-concentration FK506 (20 ng/ml) significantly inhibited the secretions of IL-2, IL-6, IL-12, IL-17, IFN-gamma, TNF-alpha, GM-CSF and G-CSF. At a moderate concentration (5 ng/ml), FK506 inhibited the secretion of GM-CSF significantly.</p><p><b>CONCLUSION</b>FK506 effectively inhibits the secretion of proinflammatory cytokines including IL-6, IFN-gamma and TNF-alpha and also the secretion of IL-2, IL-12, IL-17, GM-CSF and G-CSF. FK506 might play the role of immunosuppression by inhibiting the production of these cytokines by the immune cells. Monitoring the levels of these cytokines might be a potential method for evaluating the adequacy of FK506 doses administered.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Sangue
/
Regulação para Baixo
/
Citocinas
/
Interleucina-6
/
Interferon gama
/
Fator de Necrose Tumoral alfa
/
Tacrolimo
/
Secreções Corporais
/
Imunossupressores
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo