HPLC-MSn analysis of trigonelline and its metabolites in rat urine / 药学学报
Acta Pharmaceutica Sinica
; (12): 216-220, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-271473
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To establish a rapid and sensitive LC-MSn method for the identification of trigonelline and its main metabolites in rat urine.</p><p><b>METHODS</b>After optimizing the detection conditions of LC-MSn chromatography and mass spectrometry using trigonelline, its ionization and cleavage in ESI-MS and ESI-MSn modes were summarized, then serving as the basis for the metabolite analysis of trigonelline in rat urine. The 0-48 h urine samples of rats were collected after iv 8 mg x kg(-1) trigonelline, then, the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-MSn.</p><p><b>RESULTS</b>The structures of trigonelline metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, two phase I metabolites and the parent drug were identified existing in rat urine, and two phase II metabolites were identified.</p><p><b>CONCLUSION</b>The LC-MSn method is rapid and high sensitive and specific, it is suitable for the identification of trigonelline and its metabolites in rat urine.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Sensibilidade e Especificidade
/
Ratos Wistar
/
Espectrometria de Massas por Ionização por Electrospray
/
Trigonella
/
Alcaloides
/
Hipoglicemiantes
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Artigo