Tail-suspended model simulating mouse oocytes maturation inhibited with microgravity / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 687-696, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-271708
Biblioteca responsável:
WPRO
ABSTRACT
We studied the effects of simulated microgravity on mouse oocytes maturation, and analyzed whether the tail-suspended model can be applied to investigate simulated microgravity effects on reproductive processes in female mice. Mouse oocytes were cultured in vitro with microgravity simulated by a rotating wall vessel bioreactor and by tail-suspended model, and the maturation rate of the mouse oocytes in the two models were examined in vivo. The maturation rate of mouse oocytes cultured in simulated microgravity was 8.93%, and that was 72.33% in 1g gravity. In ratio, oocyte maturation rate had no significant difference between the rotational group and control group. Microgravity simulated by the tail-suspended model inhibited mouse oocytes maturation and increased the rate of oocytes abnormity. The maturation rate of tail-suspended mouse oocytes was 14.54%, which was significantly lower than that of control group. Tail-suspended model should be an ideal model to investigate simulated microgravity effects on reproductive processes of female mice.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oócitos
/
Oogênese
/
Fisiologia
/
Células Cultivadas
/
Simulação de Ausência de Peso
/
Elevação dos Membros Posteriores
/
Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2012
Tipo de documento:
Artigo