Crystalline nickel sulfide-induced genomic instability in transformed human broncho-epithelial cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 57-59, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-272042
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the genomic instability in the 16 human broncho-epithelial (16HBE) cells induced by crystalline nickel sulfide so as to provide the scientific basis for further study of nickel-induced cancer molecular mechanism.</p><p><b>METHODS</b>To analyse the genomic instability in transformed 16HBE cells induced by crystalline nickel sulfide by random amplified polymorphic DNA (RAPD).</p><p><b>RESULTS</b>All the 7 random primers selected could amplify 1 - 6 clear PCR bands. There were no significant differences between transformed 16HBE cells and negative control cells in the 4th, 5th, and 7th primers, but in the rest 4 primers there were significant differences, with special PCR bands for the same primer, indicating that genomic instability in transformed 16 HBE cells was induced by crystalline nickel sulfide.</p><p><b>CONCLUSION</b>Crystalline nickel sulfide could induce genomic instability in 16HBE cells.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Linhagem Celular Transformada
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
/
Cristalização
/
Instabilidade Genômica
/
Células Epiteliais
/
Toxicidade
/
Genética
/
Metabolismo
/
Níquel
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2004
Tipo de documento:
Artigo