Antisense Deoxyoligonucleotides Inhibit Activities of Matrix Metalloproteinase-2 in Human Fibrosarcoma HT1080 Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
; : 444-449, 2002.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-27225
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
MMP-2, 72 kDa-type IV collagenase, plays a major role in the migration and growth of tumor cells, a process that requires the disintegration of basement membrane. Activation of MMP-2 is correlated with the invasiveness of various tumors. The aim of this study was to determine the sequence-specific phosphorothioated oligodeoxynucleotides (ODNs) inhibiting the translation of MMP-2 mRNA and the subsequent invasiveness of tumor cells. MATERIALS ANDMETHODS:
Eight types of antisense ODNs were designed and each (8micro gram/ml) were transfected into HT1080 cells. The effects of these antisense ODNs on MMP expression were examined by gelatin zymography, Western blot, Northern blot and matrigel assay.RESULTS:
Antisense-5 (+904~923), antisense-6 (+1274~+1293) and antisense-7 (+1646~+1665) reduced the MMP-2 activity of the culture supernatant in HT1080 fibrosarcoma cells. Treatment with antisense-6 showed inhibition of MMP-2 mRNA and protein, and in vitro invasion in a dose-dependent manner.CONCLUSION:
Antisense-6 might be one of the therapeutic candidates for tumor invasion and metastasis.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligodesoxirribonucleotídeos
/
Membrana Basal
/
RNA Mensageiro
/
Western Blotting
/
Northern Blotting
/
Colagenases
/
Metaloproteinase 2 da Matriz
/
Fibrossarcoma
/
Gelatina
/
Metástase Neoplásica
Limite:
Humanos
Idioma:
Inglês
Revista:
Cancer Research and Treatment
Ano de publicação:
2002
Tipo de documento:
Artigo