Identification of A Novel HLA Allele DRB1 * 16:36 by Sequencing-Based Typing / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify a novel HLA allele DRB1 * 1636 from a Uygur woman.</p><p><b>METHODS</b>PCR-SBT technology was applied to the extracted DNA for genotyping, and a possible new gene was sequenced by using sequence specific primers and single stranded SBT. This novel allele was compared with known most homologous gene sequences and their difference was analyzed.</p><p><b>RESULTS</b>This novel allele was different from HLA alle DRB1 * 1623, and had highest similarity in 2 nucleotides at position 227 A→T and 236 T→C in exon 2, resulting in 3 amino acid changes from Tyr to Phe at codon 47 and Val to Ala at codon 50. The sequence of this novel allele had been submitted to GenBank.</p><p><b>CONCLUSION</b>This HLA allele DRB1 * 1623 has been confirmed to be a novel allele, and has been officially named DRB1 * 1636 by the World Health Organization (WHO) Nomenclature Committee in May 2015.</p>