Study on HPCE fingerprints of Poecilobdella manillensis from Guangxi province / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 495-499, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-274317
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish fingerprints for Poecilobdella manillensis from Guangxi province using the high performance capillary electrophoresis (HPCE) method.</p><p><b>METHOD</b>Electrophoresis was performed on a fused silica capillary column (75 microm x 56 cm), with 25 mmol x L(-1) Na2HPO4-120 mmol x L(-1) Tris-16 mmol x L(-1) SDS (adjusted to pH 12.0 with 1 mol x L(-1) NaOH ) as the running buffer. The applied voltage was 17 kV, the temperature was 25 degrees C and the detection wavelength was 254 nm. The sample's hydrodynamic injection was 3.4 kPa x 6s and the duration was 27 min.</p><p><b>RESULT</b>HPCE fingerprint was established with 13 common peaks. The similarity between fingerprints of P. manillensis in 10 batches and control fingerprints was more than 0.98.</p><p><b>CONCLUSION</b>The method is so precise, reproducible and stable that it could be used as a new means for the quality control of P. manillensis.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Controle de Qualidade
/
Padrões de Referência
/
Química
/
China
/
Reprodutibilidade dos Testes
/
Eletroforese Capilar
/
Sanguessugas
/
Medicina Tradicional Chinesa
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo