Determination of trigonelline in Trigonella foenum-graecum by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.</p><p><b>METHOD</b>The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(7525) and detection wavelength was set at UV 265 nm.</p><p><b>RESULT</b>The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.</p><p><b>CONCLUSION</b>It provides scientific indexes for quality control of T. foenum-graecum.</p>