Gene cloning, expression and characterization of a novel phytase from Hafnia alvei / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1017-1021, 2007.
Article
em Zh
| WPRIM
| ID: wpr-276169
Biblioteca responsável:
WPRO
ABSTRACT
A gene appA encoding a novel phytase was firstly cloned from Hafnia alvei by PCR and sequenced. The gene was consisted of 1335 bp, encoding 444 amino acids. The calculated molecular weight of the mature APPA was about 45.2 kD. The gene appA was expressed in E. coli BL21 (DE3). Recombinant APPA was purified and its enzymatic properties were determined. The optimum pH for the enzyme was 4.5 and the optimum temperature was 60 degrees C. The pH stability of r-APPA is good, the relative phytase activity was above 80% after treated in buffers of pH 2.0-10.0. The specific activity of r-APPA is 356.7 U/mg, and the Km value was 0.49 mmol/L and Vmax of 238 U/mg. The enzyme showed resistance to pepsin and trypsin treatment.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Temperatura
/
Proteínas Recombinantes
/
Dados de Sequência Molecular
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
6-Fitase
/
Hafnia
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Escherichia coli
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Genética
/
Concentração de Íons de Hidrogênio
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Article