A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 808-810, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-276345
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.</p><p><b>METHODS</b>HPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.</p><p><b>RESULTS</b>The construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.</p><p><b>CONCLUSION</b>In vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Transcrição Gênica
/
Proteínas Virais
/
RNA Polimerases Dirigidas por DNA
/
Processamento Pós-Transcricional do RNA
/
Splicing de RNA
/
Vírus da Hepatite B
/
Proteínas de Ligação a RNA
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DNA Polimerase Dirigida por DNA
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2005
Tipo de documento:
Artigo