Apoptosis of multiple myeloma RPMI8226 cell line induced by matrine / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 515-518, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-276882
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the effect of matrine on multiple myeloma RPMI8226 cell line and its mechanism. The RPMI8226 cells were treated with various concentrations of matrine for 24 and 48 hours; the promoting-apoptosis effect of matrine on RPMI8226 cells was observed by morphology, Annexin-V analysis, DNA agarose gel electrophoresis, mitochondrial transmembrane potential detection, etc. The results indicated that after treatment of RPMI8226 cells with matrine, the typical morphology of cell apoptosis appeared, Annexin-V analysis showed positive, the typical ladder was observed in DNA agarose gel electrophoresis, and the mitochondrial transmembrane potential decreased. It is concluded that the matrine can effectively inhibite the RPM8226 cell growth and reduce cell viability in dose-and time-dependent manner. It is presumed that the matrine induces RPMI8226 cell apoptosis through mitochondrial signaling pathway.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Doença Cardiovascular
/
Linfomas e Mieloma Múltiplo
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Quinolizinas
/
Apoptose
/
Linhagem Celular Tumoral
/
Relação Dose-Resposta a Droga
/
Alcaloides
/
Mieloma Múltiplo
/
Antineoplásicos Fitogênicos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo