Effects of leukemia bone marrow stromal cells on resistance of co-cultured HL-60 to idarubicin / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 163-165, 2004.
Article
em Zh
| WPRIM
| ID: wpr-278775
Biblioteca responsável:
WPRO
ABSTRACT
To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
/
Células da Medula Óssea
/
Idarubicina
/
Sobrevivência Celular
/
Células Estromais
/
Células HL-60
/
Resistencia a Medicamentos Antineoplásicos
/
Técnicas de Cocultura
/
Relação Dose-Resposta a Droga
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Article