The effects of interferon-gamma on the expression of the cyclin D isoforms in cord blood hematopoietic stem/progenitor cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 138-141, 2004.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-278781
Biblioteca responsável:
WPRO
ABSTRACT
To explore the hematopoiesis inhibition mechanisms of interferon-gamma (IFN-gamma), the effects of IFN-gamma on the expression of the cyclin D in the umbilical cord blood hematopoietic stem/progenitor cells were observed. In the experiments the CD34(+) cells were isolated from the cord blood with MIDI-MACS system; semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; the expression levels of cyclin D isoforms were assayed by semi-quantitative RT-PCR, after the hematopoietic stem/progenitor cells were incubated with IFN-gamma. The results indicated that IFN-gamma could inhibit the formation of CFU-GM and down-regulate the expression of cyclin D2 and cyclin D3 at the mRNA level. It is concluded that the IFN-gamma could inhibit the proliferation of hematopoietic stem cells and down-regulate the expression of cyclin D, that may be one mechanism underlying the hematopoietic inhibition of IFN-gamma.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
RNA Mensageiro
/
Células-Tronco Hematopoéticas
/
Fase G1
/
Interferon gama
/
Ciclinas
/
Isoformas de Proteínas
/
Biologia Celular
/
Ciclina D
/
Sangue Fetal
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo