Coxsackie virus B types were discriminated by RT-PCR / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 291-293, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-279550
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.</p><p><b>METHODS</b>A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.</p><p><b>RESULTS</b>This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.</p><p><b>CONCLUSION</b>The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Sangue
/
Imunoglobulina M
/
Ensaio de Imunoadsorção Enzimática
/
Classificação
/
Primers do DNA
/
Enterovirus Humano B
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Diagnóstico
/
Infecções por Enterovirus
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2004
Tipo de documento:
Artigo