Characterization of the whole genome from a human parechovirus type 3 detected from the serum of a child with sepsis in Beijing, China / 病毒学报
Chinese Journal of Virology
; (6): 541-548, 2014.
Article
em Zh
| WPRIM
| ID: wpr-280330
Biblioteca responsável:
WPRO
ABSTRACT
Human parechovirus type 3 (HPeV3) is an important pathogen of severe sepsis. HPeV3 is a non- enveloped, single-stranded, positive-sense RNA virus with a linear and continuous genomic RNA. The complete genome of a HPeV3 (BJ-C3174) strain was analyzed from the serum specimen from a child with sepsis hospitalized in Beijing, China, in 2012. The whole genome of BJ-C3174 was 7329 nucleotides (nt) in length excluding a poly (A) tail. One large open reading frame (ORF) of 6531 nt encoding a putative polyprotein precursor of 2177 amino acids (aa) was flanked by a 5' untranslated region (UTR) of 709 nt and 3' UTR of 91 nt. Phylogenetic analysis showed that BJ-C3174 belonged to HPeV3 and was closest to the HPeV3 strain BONN-2 from Germany. Compared with HPeV1-8 reference strains, BJ-C3174 shared the highest similarities with BONN-2 in full length and in each of the gene segments of the genome. The nucleotide and predicted amino acid identities of the whole genome between BJ-C3174 and BONN-2 were 99.3% and 99.8%, respectively, which were higher than those compared with HPeV3 prototype. Recom- bination of the gene segment with other HPeVs types was not identified.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Virologia
/
Sangue
/
Dados de Sequência Molecular
/
Sequência de Aminoácidos
/
Classificação
/
Genoma Viral
/
Sepse
/
Parechovirus
/
Genética
Limite:
Child
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2014
Tipo de documento:
Article