Construction of pEGFP-C1/U6-mediated plasmid expressing MDR1 shRNA / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 384-387, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-280660
Biblioteca responsável:
WPRO
ABSTRACT
To construct a plasmid expressing MDR1 short hairpin RNA (shRNA) mediated by pEGFP-C1/U6 vector, two coding sequences of 19 nucleotides were selected from MDR. Two pairs of oligonucleotides were designed for these two fragments. After annealing the formed double-stranded DNAs were ligated with plasmid pEGFP-C1/U6 (pEGFP-C1 vector with U6 promoter). The plasmids producing MDR1 shRNA were constructed from the inverted motif containing 9 spacers and four Ts. The results showed that the constructed plasmids were named pEGFP-C1/U6/A and pEGFP-C1/U6/B, and the constructs were identified by restriction and sequence analysis, no any base mutation was observed. It is concluded that plasmids of pEGFP-C1/U6/A and pEGFP-C1/U6/B expressing MDR1 shRNA were successfully constructed, providing a highly effective method for reversing the multidrug resistance in clinic.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
RNA Mensageiro
/
RNA Nuclear Pequeno
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Resistência a Múltiplos Medicamentos
/
Resistencia a Medicamentos Antineoplásicos
/
RNA Interferente Pequeno
/
Proteínas de Fluorescência Verde
/
Proteínas de Ligação a DNA
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo