Preparation and characterization of a monoclonal antibody against human SOCS3 / 南方医科大学学报
Journal of Southern Medical University
; (12): 1778-1780, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-281539
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare and characterize the monoclonal antibody (mAb) against human SOCS3.</p><p><b>METHODS</b>BALB/c mice were immunized with recombinant GST-SOCS3 protein, from which the spleen cells were isolated and fused with Sp2/0 cells. After several rounds of screening and cloning, the hybridoma cell strain secreting anti-SOCS3 mAb was obtained, whose specificity was evaluated using ELISA and Western blotting, and the titer, immunoglobulin subtype and affinity of the mAb were also measured.</p><p><b>RESULTS</b>The hybridoma cell strain secreting anti-SOCS3 mAb was identified to belong to IgG2a subtype. The mAb titers in cultural supernatant and acetic fluid were 1640 and 125600, respectively, as determined by ELISA with affinity reaching 4.84x10(6) L/mol.</p><p><b>CONCLUSION</b>The success in anti-SOCS3 mAb preparation provides the basis for further study of the negative regulation of cytokine signal transduction and the immunoregulation in microorganism infections.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Secreções Corporais
/
Alergia e Imunologia
/
Proteínas Supressoras da Sinalização de Citocina
/
Proteína 3 Supressora da Sinalização de Citocinas
/
Hibridomas
/
Camundongos Endogâmicos BALB C
/
Anticorpos Monoclonais
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo