Effects of conjugated linoleic acid on the expression of critical enzymes of linoleic acid metabolism in tumor cell / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 195-198, 2005.
Article
em Zh
| WPRIM
| ID: wpr-282361
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on critical enzymes of linoleic acid metabolism in stomach granular cell (SGC-7901).</p><p><b>METHODS</b>SGC-7901 was treated with c9,t11-CLA by 200, 100, 50 or 25 micromol/L for 24 hours. The effects of c9,t11-CLA on the cell proliferation was measured by monotetrazolium and the expression of Delta6-desaturase, Delta5-desaturase, COX-1, COX-2, 5-LOX mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>At a concentration of 200, 100, 50, or 25 micromol/L, c9,t11-CLA suppressed the proliferation of SGC-7901 by 54.3%, 20.5%, 10.5% and 2.93%. The c9,t11-CLA might decrease the expression of COX-2 mRNA, and increase the expression of Delta6-desaturase and COX-1 in SGC-7901, but might not affect Delta5-desaturase and 5-LOX.</p><p><b>CONCLUSION</b>The effects of c9,t11-CLA on the COX and Delta6-desaturase might play an important role in mediating the ability of c9,t11-CLA as to inhibiting the proliferation of tumor cells, and the anti-cancer activity by c9,t11-CLA might be associated with the linoleic acid metabolism.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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RNA Mensageiro
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Ácidos Linoleicos
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Regulação Enzimológica da Expressão Gênica
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Regulação Neoplásica da Expressão Gênica
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Lipoxigenase
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Perfilação da Expressão Gênica
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Linhagem Celular Tumoral
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Ácidos Linoleicos Conjugados
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2005
Tipo de documento:
Article