Effects of diethylhexyl phthalate on lipid peroxidation and the life-span in Drosophila melanogaster / 中华预防医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effects of diethlhexyl phthalate (DEHP) on lipid peroxidation and the life span in Drosophila melanogaster.</p><p><b>METHODS</b>Fed Drosophila with the concentration 0.20% DEHP of exposure after 0, 14, 28 days, the activity of total superoxide dismutase (SOD), CuZn-SOD and the concentration of malondialdehyde were determined. At the same time, the longevity test was carried out to examine the effect of DEHP on the Drosophila's lifespan.</p><p><b>RESULTS</b>The lifespan of Drosophila was shortened in a dose of DEHP exposed groups. The indexes of mean life span (MLS), 50% lethal time and mean maximum life span in three DEHP-treated groups (concentration of 0.05%, 0.10% and 0.20%) were lower than those of the controlled group respectively (P < 0.01 or P < 0.05). The MLS of both Drosophila sexes were reduced from the control of 64 days and 59 days to the test 60 days-52 days and 54 days-49 days respectively. DEHP decreased the activity of SOD (P < 0.01 or P < 0.05), and lead to a time-dependent relation and an increase in the concentration of malondialdehyde (P < 0.01 or P < 0.05) in the DEHP-exposed Drosophila groups.</p><p><b>CONCLUSION</b>DEHP might promote the process of lipid peroxidation and shorten the life span in Drosophila melanogaster. It should be one of the reasons in the senescence of Drosophila.</p>