Isolation and physiological characterization of mesenteric arterial smooth muscle cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 954-958, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-282877
Biblioteca responsável:
WPRO
ABSTRACT
Vascular smooth muscle cells (VSMCs) are crucial for studying the mechanism and regulation of vasogenic response, but so far no ideal method has been reported for their isolation. We established a VMSC isolation method based on two-step digestion, with which a large population of single smooth muscle cells could be obtained. After cell isolation, the potassium current, cytosolic calcium concentration, reactivity to caffeine were measured, and the results demonstrated that the cells were highly viable and exhibited reactivity to the vascular constrictor to meet various needs in experimental research.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Canais de Potássio
/
Separação Celular
/
Cálcio
/
Ratos Wistar
/
Técnicas de Patch-Clamp
/
Biologia Celular
/
Miócitos de Músculo Liso
/
Potenciais da Membrana
/
Artérias Mesentéricas
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo