Expression of HSF1 and XAF1 in gastro-intestinal cancer / 南方医科大学学报
Journal of Southern Medical University
; (12): 1447-1450, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-283109
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>X-linked inhibitor of apoptosis protein (XIAP) To gastrointestinal (GI) investigate the expression of XAF1 and heat-shock transcription factors 1 èHSF1éand their relationship in human gastrointestinal cancers.</p><p><b>METHODS</b>Immunoblotting was used to analyze the expression of HSF1 and XAF1 in either gastric or colon cancer tissue and GI cancer cell line. Transient expression of the HSF1-containing vector in GI cell lines and RNA interference were used to up/down-regulae the expression of the HSF1, and the subsequent expression of XAF1 was measured.</p><p><b>RESULTS</b>The expression of HSF1 was higher in GI cancers than in normal tissues. The expression of XAF1 and HSF1 was negatively correlated in GI cancer cell lines. Stress stimuli up-regulated the expression of HSF1 while the alteration of XAF1 expression was negatively correlated with HSF1 expression.</p><p><b>CONCLUSION</b>The high expression of HSF1 in GI cancers is associated with suppressed expression of XAF1, which can be one of the mechanisms for low-expression of XAF1 and insufficient apoptosis in GI cancers.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Temperatura
/
Fatores de Transcrição
/
Sequência de Bases
/
Regulação Neoplásica da Expressão Gênica
/
Estresse Oxidativo
/
Linhagem Celular Tumoral
/
Peptídeos e Proteínas de Sinalização Intracelular
/
Proteínas de Ligação a DNA
/
Fatores de Transcrição de Choque Térmico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo