Tissue culture and polyploidy induction of Morinda officinalis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2325-2328, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-283202
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an effective protocol for plant generation and induce polyploidy of Morinda offcinalis.</p><p><b>METHOD</b>Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry.</p><p><b>RESULT AND CONCLUSION</b>The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg x L(-1) colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Poliploidia
/
Cromossomos de Plantas
/
Morinda
/
Técnicas de Cultura de Tecidos
/
Genética
/
Métodos
Tipo de estudo:
Guia de prática clínica
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Artigo