Value of lymph node micrometastasis detected by RT-PCR assay in determining the stage of gastric carcinoma / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 217-220, 2006.
Article
em Zh
| WPRIM
| ID: wpr-283353
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect micrometastasis in regional lymph nodes using RT- PCR assay and evaluate the significance of the new assessment of nodal status in determining pN staging in gastric carcinoma.</p><p><b>METHODS</b>In addition to HE,RT- PCR assay for cytokeratin-20 gene marker was used to detect micrometastasis in 850 lymph nodes from 30 patients with gastric carcinoma who underwent gastrectomy with lymphadenectomy from Dec. 2003 to Apr. 2004.</p><p><b>RESULTS</b>The detection rate of HE staining was 27.1% (233/850), the detection rate of RT-PCR assay was 36.5% (310/850) (Pa< 0.01). Lymph node micrometastasis was further detected in 77 nodes from 14 patients. The detection rate of RT- PCR assay from the routine negative lymph nodes was 12.5% (77/617). Seven of those patients were up- staged (from IB stage to II stage, from IB stage to III A stage, from I stage to III A stage, from III A stage to III B stage, from III A stage to IV stage in one patient respectively, and from III B stage to IV stage in 2 patients).</p><p><b>CONCLUSION</b>RT- PCR assay can increase the detection rate of lymph node metastasis and have a significant impact on the staging system of gastric carcinoma.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Neoplasias Gástricas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Linfonodos
/
Metástase Linfática
/
Estadiamento de Neoplasias
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2006
Tipo de documento:
Article