Study on fingerprints of Citrus aurantium from different places by capillary electrophoresis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.</p><p><b>METHOD</b>The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.</p><p><b>RESULT</b>There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.</p><p><b>CONCLUSION</b>The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.</p>