Protective effects of hepatocyte growth factor on hypoxic human pulmonary microvascular endothelial cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 1-5, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-284314
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of hepatocyte growth factor (HGF) on hypoxic human pulmonary microvascular endothelial cells (HPMECs).</p><p><b>METHODS</b>HPMECs were cultured in vitro, and the hypoxic model was established by the physical method. Cells were divided into 4 groups the control group, the hypoxic group, HGF group, and phytohemagglutinin (PHA) group. The 7(th) generation of HPMECs was evaluated by the method of immunocytochemistry. The persistence rate of HPMECs was measured by MTT assay and the adhesive cells were counted by the microscopy. The expression of intercellular adhesion molecule-1 (ICAM-1) protein was determined by immunofluorescence staining.</p><p><b>RESULTS</b>The adherence percentage of cells significantly decreased after hypoxia, whereas the expression of the ICAM-1 protein was significantly higher in the hypoxia group than in control group (P<0.01). Compared with the hypoxia group, the persistence and adherence percentage of cells in the HGF group significantly increased (P<0.01), whereas the expression of the ICAM-1 protein significantly dropped (P<0.01). In the PHA group, the persistence and adhesion rate were significantly different from those in the hypoxia group and HGF group (P<0.01), and the expression of the ICAM-1 protein increased significantly (P<0.01).</p><p><b>CONCLUSION</b>HGF could inhibit the hypoxic damage of HPMECs by decreasing the persistence and the adhesive capacity of these cells and inducing the expression of ICAM-1.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Endotélio Vascular
/
Hipóxia Celular
/
Adesão Celular
/
Sobrevivência Celular
/
Células Cultivadas
/
Fator de Crescimento de Hepatócito
/
Molécula 1 de Adesão Intercelular
/
Biologia Celular
/
Células Endoteliais
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2013
Tipo de documento:
Artigo