Study on the differentially expressed genes in bone tissue of ovariectomized rats after intervention with Chinese herbs / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 138-142, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-284493
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the therapeutic effect and mechanism of jiangu granule (JGG) on postmenopausal osteoporosis.</p><p><b>METHODS</b>Differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.</p><p><b>RESULTS</b>Ten differentially expressed gene fragments were found, 9 of them, after cloning, sequencing and BLAST searching, were proved to be matched with highly homologous sequences in Genebank. Among them, two were known proteins hyaluronan-mediated motility receptor (RHAMM) and ATPase, Na+, K+ transporting beta 3 polypeptide (ATP1b3). It was confirmed by semi-quantitative RT-PCR that these two gene fragments could be down- and up-regulated by JGG respectively.</p><p><b>CONCLUSION</b>The therapeutic effect of JGG in treating postmenopausal osteoporosis might be related with its regulation on the two kinds of protein, RHAMM and ATP1b3.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoporose
/
Farmacologia
/
Osso e Ossos
/
Medicamentos de Ervas Chinesas
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Ovariectomia
/
Regulação da Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Ratos Sprague-Dawley
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Perfilação da Expressão Gênica
/
Tratamento Farmacológico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo