Expressions of neuropathic pain-related proteins in the spinal cord dorsal horn in rats with bilateral chronic constriction injury / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 628-633, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-285948
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the pain-related behavioral changes in rats with bilateral chronic constriction injury(bCCI)and identify the expressions of neuropathic pain-related proteins.</p><p><b>METHODS</b>The bCCI models were established by ligating the sciatic nerves in female Sprague Dawley rats. Both mechanical hyperalgesia and cold hyperalgesia were evaluated through electronic von Frey and acetone method. Liquid chromatography-mass spectrometry/mass spectrometry was applied to characterize the differentially expressed proteins.</p><p><b>RESULTS</b>Both mechanical withdrawal threshold and cold hyperalgesia threshold decreased significantly on the postoperative day 7 and 14, when compared with na ve or sham rats(P <0.05). Twenty five differentially expressed proteins associated with bilateral CCI were discovered, with eighteen of them were upregulated and seven of them downregulated.</p><p><b>CONCLUSIONS</b>The bCCT rats have remarkably decreased mechanical and cold hyperalgesia thresholds. Twenty five neuropathic pain-related proteins are found in the spinal cord dorsal horn.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dor
/
Nervo Isquiático
/
Medula Espinal
/
Ratos Sprague-Dawley
/
Limiar da Dor
/
Constrição
/
Corno Dorsal da Medula Espinal
/
Hiperalgesia
/
Metabolismo
/
Neuralgia
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2013
Tipo de documento:
Artigo