Screening and characterization of lipase from a metagenome library of dairy rumen microflora / 生物工程学报
Chinese Journal of Biotechnology
; (12): 869-874, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-286630
Biblioteca responsável:
WPRO
ABSTRACT
Using lipase segregation agar containing trioleoylglycerol, we obtained 18 lipase positive clones by screening from a metagenome library of dairy rumen microflora containing 15,360 clones. The average insert size of lipase positive clones was about 60 kb. Lipase enzyme activity analysis by p-NPP method indicated that Lipase6, Lipase7 and Lipase8 had higher lipolytic activities to substrates of p-nitrophenyl palmitate (C16), p-nitrophenyl alaurate (C12) and p-nitrophenyl palmitate (C16) respectively. The optimum pH of Lipase 6, Lipase 7 and Lipase 8 were 7.5. The halflife period of Lipase 8 with the value of 15 min in 70 degrees C decreased with the increase of temperature. In conclusion, the lipases screened in this study had different substrates specificity and good thermo stability, which laid a basis for large-scale industrial application.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rúmen
/
Especificidade por Substrato
/
Temperatura
/
Bactérias
/
Biblioteca Gênica
/
Clonagem Molecular
/
Metagenoma
/
Genética
/
Lipase
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo