Extract of Zuojin Pill (characters: see text) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 837-845, 2015.
Article
em En
| WPRIM
| ID: wpr-287180
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.</p><p><b>METHODS</b>Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.</p><p><b>RESULTS</b>ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.</p><p><b>CONCLUSIONS</b>ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Medicamentos de Ervas Chinesas
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Sobrevivência Celular
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Western Blotting
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Espécies Reativas de Oxigênio
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Apoptose
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Colorimetria
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Ensaio Cometa
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Linhagem Celular Tumoral
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Membranas Mitocondriais
Limite:
Humans
Idioma:
En
Revista:
Chinese journal of integrative medicine
Ano de publicação:
2015
Tipo de documento:
Article