Determination of three alkaloids in total alkaloids exfracts from Lotus plumule by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule.</p><p><b>METHOD</b>A Hypersil C18 column was used with the mobile phase of methanol-phosphate (7327, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1).</p><p><b>RESULT</b>The linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol.</p><p><b>CONCLUSION</b>The method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.</p>