Antimicrobial activity of endophytic fungi isolated from Dendrobium species in southwestern China / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 764-770, 2012.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-288710
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and characterize endophytic fungi from seven Dendrobium species, and detect their antimicrobial activities.</p><p><b>METHOD</b>Fungal endophytes were isolated by strictly sterile sample preparation and fungal identification methods were based on their ITS ribosomal DNA (ITS rDNA gene) sequences. The agar well diffusion method was then employed to evaluate the antimicrobial activity against six pathogenic organisms and the phylogenetic tree of active isolates was constructed by the MEGA.</p><p><b>RESULT</b>Ninety-eight endophytic fungi obtained from seven Dendrobium spp., and among them twenty-four isolates, representing 11 genera and 14 species, displayed anti-microbial activities. The phylogenetic assay based on ITS-rDNA showed that 24 active isolates were sorted to 7 taxonomic orders Hypocreales, Sordariales, Capnodiales, Eurotiales, Botryosphaeriales, Xylariales and Mucorales. The results of antimicrobial activity assay revealed that 1.02%, 10.2%, 18.4%, 1.02%, 1.02% and 10.2% of fermentation broths of 98 isolates displayed significant antimicrobial activities against E. coli, B. subtilis, S. aureus, C. albicans, C. neoformans and A. fumigatus, respectively. Four strains DL-R-3, DL-S-6, DG-R-10 and DN-S-1 displayed strong and broad antimicrobial spectrum.</p><p><b>CONCLUSION</b>Endophytic fungi associated with Dendrobium species have fungal diversity, and possess diverse antimicrobial activity.</p>
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Filogenia
/
Fisiologia
/
Aspergillus fumigatus
/
Staphylococcus aureus
/
Bacillus subtilis
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Candida albicans
/
DNA Fúngico
/
Dados de Sequência Molecular
/
Sequência de Bases
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo