Immortalized human odontoblast-like cell line expressed dentin extracellular matrix in vitro / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 518-521, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-289026
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro.</p><p><b>METHODS</b>Collagen type I, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP) were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week.</p><p><b>RESULTS</b>DSPP, DMP1, BSP and collagen type I were expressed in hTERT-hOd-1 either at mRNA or protein level. Under the induction of mineralizing medium, the cells showed higher activity of ALP and increased secretion of OC.</p><p><b>CONCLUSION</b>hTERT-hOd-1 expressed dentin extracellular matrix in vitro, which means the cell line has the potential of mineralization.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfoproteínas
/
Sialoglicoproteínas
/
Técnicas In Vitro
/
RNA Mensageiro
/
Imuno-Histoquímica
/
Linhagem Celular
/
Proteínas da Matriz Extracelular
/
Hibridização In Situ
/
Colágeno Tipo I
/
Dentina
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2005
Tipo de documento:
Artigo