An experimental study on the repair of full skin loss of rabbits with composite chitosan artificial skin / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the practicability of repair of full skin loss of rabbits with composite chitosan artificial skin.</p><p><b>METHODS</b>Dermal substitute was prepared aseptically by mixing fibroblasts with composite dermal matrix gel. Keratinocytes were then seeded on the substitute and submersion - cultured thereafter for 1 week in keratinocyte culture medium. The composite was further cultured for 1 approximately 2 weeks on the surface of the culture liquid to form artificial skin. The composite chitosan artificial skin was then grafted onto the full skin loss wound of rabbits. Histological changes were undertaken periodically by tissue sampling from the grafted wound. The systemic reaction of rabbits to the artificial skin was observed.</p><p><b>RESULTS</b>All the grafted wounds healed very well without any suppuration, bleeding or infection under the grafted skin. No obvious immune rejection was seen. The artificial skin could cover the wounds for a long time with good elasticity and easy to be manipulation.</p><p><b>CONCLUSION</b>The composite chitosan artificial skin could be an optimal biological dressing with good histocompatibility and easy to be manipulation.</p>