Effects of Survivin-T34A mutant on breast cancer cell in vitro and in vivo / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 648-651, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-290699
Biblioteca responsável:
WPRO
ABSTRACT
To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the experiment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Survivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9-null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow cytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47.1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Neoplasia da Mama
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias da Mama
/
Transfecção
/
Injeções Intralesionais
/
Apoptose
/
Tratamento Farmacológico
/
Proteínas Inibidoras de Apoptose
/
Genética
/
Camundongos Nus
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2014
Tipo de documento:
Artigo