Report of a case of congenital plasminogen activator inhibitor-1 deficiency / 中华血液学杂志
Chinese Journal of Hematology
; (12): 129-131, 2004.
Article
em Zh
| WPRIM
| ID: wpr-291434
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To report a patient with congenital plasminogen activator inhibitor-1 (PAI-1) deficiency and explore its molecular mechanism.</p><p><b>METHODS</b>The activities of tissue plasminogen activator (tPA), alpha(2) antiplasmin (alpha(2)AP) and PAI-1 were measured by the methods of chromogenic substrate, the antigens of tPA and PAI-1 were measured by ELISA. PAI-1 gene was studied by PCR product sequencing and restriction endonuclease ana-lysing.</p><p><b>RESULTS</b>In the present patient, the euglobulin clot lysis time was 70 minutes and was corrected to normal range after added 50 ng/ml PAI-1 to his plasma. The activities of t-PA, alpha(2)AP, and factor were normal; the activity and antigen of PAI-1 in plasma were both significantly decreased. Nucleotide sequence analysis revealed that the patient had a heterozygous missense mutation in exon 2, a G to A transition at nucleotide 43. The possibility of gene polymorphism was excluded by restriction endonuclease analysing.</p><p><b>CONCLUSIONS</b>It is the first patient with congenital PAI-1 deficiency reported in China. The PAI-1 deficiency in the patient may be caused by compound heterozygosity, one of which is the G to A transition at nt43, a new mutation in congenital PAI-1 deficiency.</p>
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Base de dados:
WPRIM
Assunto principal:
Sangue
/
Dados de Sequência Molecular
/
Sequência de Bases
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Inibidor 1 de Ativador de Plasminogênio
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Genética
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Mutação
Limite:
Adult
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2004
Tipo de documento:
Article