Reversal of multi-drug resistance in K562/A02 cells by small interference RNA of mdr1 gene / 中华血液学杂志
Chinese Journal of Hematology
; (12): 5-7, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-291463
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of small interference RNA (siRNA) on mdr1 and P-glyco-protein (P-gp) expression of multi-drug resistance (MDR) human leukemia cell line K562/A02.</p><p><b>METHODS</b>Three si RNAs (si-mdr1-1, si-mdr1-2, si-mdr1-3) which were specifically targeted mdr1 gene were synthesized and transfected into K562/A02 cells. Expression of mdr1 mRNA was assayed by RT-PCR. P-gp expression and intracellular daunorubicin (DNR) concentration were determined by flow cytometry. 50% inhibition concentration (IC(50)) of doxorubicin (ADM) on K562/A02 was determined by MTT method.</p><p><b>RESULTS</b>Treatment of K562/A02 cell with the 3 kinds of siRNAs resulted in a reversal of MDR of a different extent. The third siRNA was more effective in the suppression of mdr1 with a significant reduction of (58.0 +/- 1.54)% of the mdr1 mRNA expression. Positive expression rate of p170 decreased from (76.0 +/- 1.0)% to (19.6 +/- 1.9)%, and the relative efficiency of K562/A02 to ADM was 70.4%. The intracellular accumulation of DNR increased after siRNA treatment.</p><p><b>CONCLUSION</b>The siRNA could effectively restore the sensitivity of K562/A02 cells to conventional chemotherapeutic agents.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
RNA Mensageiro
/
Dados de Sequência Molecular
/
Farmacocinética
/
Sequência de Bases
/
Daunorrubicina
/
Resistencia a Medicamentos Antineoplásicos
/
Genes MDR
/
Células K562
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo