An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF.</p><p><b>METHODS</b>Cultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c-myc mRNA expression were detected.</p><p><b>RESULTS</b>The expression of c-myc mRNA in the bFGF-treated groups increased (P < 0.05) with a dose- and stimulating time-dependent manner. The c-myc mRNA expression reached to a peak level at 2 hours.</p><p><b>CONCLUSIONS</b>C-myc expression may be an important component in controlling the transit of cells through the cell cycle.</p>