Novel mutations of cathepsin C gene in two Chinese patients with Papillon-Lefèvre syndrome / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 602-605, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-293036
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mutational characteristics of cathepsin C (CTSC) gene in two Chinese patients with Papillon-Lefèvre syndrome (PLS), and provide molecular basis for research of the pathogenesis of PLS.</p><p><b>METHODS</b>Peripheral blood samples were obtained from patients and their parents respectively. Genomic DNA were extracted after consents. Polymerase chain reaction, direct DNA sequencing and restriction enzyme reaction were performed to screen mutations of CTSC gene.</p><p><b>RESULTS</b>Compound heterozygous mutations of CTSC gene were identified in the two patients. Patient I carried the G139R and S260P mutations, patient II had the R250X and C258W mutations. The parents were heterozygous carriers without the clinical feature of PLS. None of the mutations were detected in normal controls. Furthermore, the S260P and C258W changes were novel mutations of CTSC gene, which had not been reported previously.</p><p><b>CONCLUSIONS</b>Mutations of CTSC gene are responsible for the phenotype of Papillon-Lefèvre syndrome in two Chinese patients. The results extend the mutation spectrum of CTSC gene and also provide basis for gene diagnosis of PLS in China.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doença de Papillon-Lefevre
/
Éxons
/
Mutação de Sentido Incorreto
/
Catepsina C
/
Povo Asiático
/
Genética
Limite:
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2006
Tipo de documento:
Artigo