HPLC fingerprints establishment of chemical constituents in genus Chimonanthus leaves / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1560-1563, 2013.
Article
em Zh
| WPRIM
| ID: wpr-294068
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to establish the HPLC fingerprints of the genus Chimonanthus leaves and compare the constituents distribution among five Chimonanthus species . The analysis was conducted on a C15 column (4.6 mm x 250 mm, 5 microm) with the mobile phase containing acetonitrile-water in gradient program: acetonitrile (B), 0-20 min, 6%-20%; 20-30 min, 20%-25%; 30-40 min, 25%-45%; 40-50 min, 45%-80%; 50-80 min, 80%-85%; 80-90 min, 85%-100%; 90-110 min, 100%. Flow rate was 0.8 mL x min(-1) and detection wavelength was 228 nm. Column temperature was set at 30 degrees C. The HPLC fingerprints of the five Chimonanthus species have been established. Ch. praecox, Ch. nitens, Ch. salicifolius, Ch. Zhejiangensis and Ch. grammatus have significant difference in constituents distribution and contents. Five standard substances as common compounds were confirmed in chromatography fingerprints. The method can be used as quality evaluation and classicfication of the genus Chimonanthus.
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Folhas de Planta
/
Calycanthaceae
/
Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Article