Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS(n) / 药学学报
Acta Pharmaceutica Sinica
; (12): 1004-1009, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-294898
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.</p><p><b>METHODS</b>Urine samples from 0 - 24 h were collected after single ig dose of 500 mg x kg(-1) daidzein to each of six rats. The urine samples were purified by SPE column (SPE C18) and analyzed with liquid chromatographic-tandem electrospray ionization ion trap mass spectrometry (LC-ESI/MS(n)) for potential metabolites.</p><p><b>RESULTS</b>Several new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine.</p><p><b>CONCLUSION</b>LC-ESI/MS(n) is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sementes
/
Glycine max
/
Sulfatos
/
Urina
/
Estrutura Molecular
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida
/
Ratos Sprague-Dawley
/
Espectrometria de Massas por Ionização por Electrospray
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Artigo