Liquid chromatography-tandem electrospray ionization ion trap mass spectrometric assay for the metabolites of jatrorrhizine in rat urine / 药学学报
Acta Pharmaceutica Sinica
; (12): 846-851, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-294927
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To identify the main metabolites of jatrorrhizine in rat urine.</p><p><b>METHODS</b>The rat urine samples were collected 0 - 72 h after ig 12 mg x kg(-1) jatrorrhizine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by combining liquid chromatography and tandem electrospray ionization ion trap mass spectrometry (LC-ESI/ITMS(n)). Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug.</p><p><b>RESULTS</b>At least seven phase I metabolites (such as de-methyl, de-hydrogen and hydroxyl metabolites) and eleven phase II metabolites (such as glucuronide conjugates and methyl-conjugates) were identified in rat urine.</p><p><b>CONCLUSION</b>The developed LC-ESI/ITMS(n) method is not only simple and rapid but also sensitive and specific for the identification of metabolites of jatrorrhizine in rat urine.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Urina
/
Berberina
/
Estrutura Molecular
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Ratos Wistar
/
Espectrometria de Massas por Ionização por Electrospray
/
Coptis
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Artigo