Effects of rat serum containing total flavonoid extract of Epimedium sagittatum on development of osteoblasts / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 928-931, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-295437
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of total flavonoid extract of Epimedium sagittatum (TFE) on the proliferation and differentiation of newborn rat calvarial osteoblasts (ROB).</p><p><b>METHOD</b>TFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100 microg x mL(-1) respectively. The serum of rats administered TFES (SRAT) was also added into the medium in a parallel treatment at 2.5%, 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MTT and the analysis of osteogenic differentiation marks.</p><p><b>RESULT</b>TFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2.5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures.</p><p><b>CONCLUSION</b>The metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Flavonoides
/
Sangue
/
Calcificação Fisiológica
/
Medicamentos de Ervas Chinesas
/
Diferenciação Celular
/
Química
/
Cálcio
/
Ratos Wistar
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2008
Tipo de documento:
Artigo