Effect of total glucosides of paeony on nuclear factor-kappaB activation in rat peritoneal macrophages / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 669-671, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-295461
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS)-induced nuclear factor-kappaB (NF-kappaB) activation in macrophages.</p><p><b>METHOD</b>Rat peritoneal macrophages were pre-treated with TGP for 2 h and stimulated with LPS for 20 min or 0.5 h. Inhibitory kappaBalpha (IkappaBalpha) protein in the cytoplasm and NF-kappaB p65 protein in the nuclear were analyzed by western blot. Further, DNA binding activity of NF-kappaB complex was detected.</p><p><b>RESULT</b>TGP enhanced the amounts of IkappaBalpha protein in the cytoplasm and decreased the amounts of NF-kappaB p65 protein in the nuclear of LPS-induced macrophages. TGP also inhibited the LPS-mediated DNA binding activity of NF-kappaB complex in macrophages.</p><p><b>CONCLUSION</b>TGP can inhibit LPS-induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
DNA
/
Núcleo Celular
/
Química
/
NF-kappa B
/
Macrófagos Peritoneais
/
Transporte Proteico
/
Paeonia
/
Biologia Celular
/
Citoplasma
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2008
Tipo de documento:
Artigo