Clinical and experimental study of a multiple myeloma case with low hypodiploidy / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 343-346, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-295480
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To report the clinical and laboratory characterization of a case of multiple myeloma with low hypodiploid complex karyotyptic abnormalities.</p><p><b>METHODS</b>Cytogenetic examination of bone marrow performed by 24 h culture method. R-banding technique was used to analyze the karyotype. Interphase fluorescence in situ hybridization (FISH) was performed using chromosome probes such as 13q14, p53, Rb1, 1q21 and IgH/CCND1. The DNA content was detected by flow cytometry.</p><p><b>RESULTS</b>Chromosome analysis revealed complex chromosomal rearrangement. Five cells had a low hypodiploid karyotype with 35 chromosomes. Three cells had the duplication of the low hypodiploid karyotype. Four cells had a normal karyotype. Monosomy 1, 13, 14, 17 and a mark chromosome 1 derived from chromosome 11 resulting in the amplication of CCND1 gene were confirmed by interphase FISH. Flow cytometric analysis displayed a low hypodiploid peak with the DNA index of 0.8426.</p><p><b>CONCLUSION</b>These results indicated that the low hypodiploidy is a rare abnormality in multiple myeloma. Interphase FISH is a reliable method for detecting molecular abnormalities in multiple myeloma.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rearranjo Gênico
/
Citogenética
/
Diagnóstico
/
Cariótipo Anormal
/
Genética
/
Métodos
/
Mieloma Múltiplo
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2012
Tipo de documento:
Artigo